Consider Chrysin. “so the chrysin can only become chemotherapy sensitizer.”
Chrysin Reverse the Drug Resistance by Targeting Breast Cancer Stem Cell Related Subpopulation
In the presence of chrysin (2.5μM,5μM), the IC50 value of mitoxantrone in MCF-7 cells was not significantly changed .Conclusions: 1、The MCF-7/MX cell line can be constructed by stepwise selection in mitoxantrone . 2、After being induced by mitoxantrone, MCF-7/MX cells is highly drug-resistant .3、
Chrysin can reverse drug resistance of MCF-7/MX cells by targeting BCRP.
Part Two : Chrysin influences breast cancer stem cell Subgroup in MCF-7/MX cell lines
Objective: To Investigate the influence of chrysin on CD44~+CD24~- cell in MCF-7/MX cell linesMethods: We used FACSC and Cell immunofluorescence to analyse the percentage of CD44~+ CD24~- cells in MCF-7 and MCF-7/MX cell lines.Results: Nearly 95% cells of MCF-7/MX and MCF-7 cell is CD44~+CD24~+. The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was was not significantly changed by only Chrysin treatment relative to the CD44~+CD24~-cells in untreated cells (P > 0.05).The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was decreased by Chrysin and Mitoxantrone treatment relative to the CD44~+CD24~-cells found at 3.2% in untreated cells. In MCF-7/MX cells treated by Chrysin and Mitoxantrone, the CD44~+CD24~-cells population was 2.684±0.226%,1.907±0.179 %,1.414±0.112 % and 1.448±0.057% at 10-2μM、10-1μM、100μM、101μM Chrysin , respectively(P < 0.05).
The same results could be Investigate by using Cell immunofluorescence.Conclusions: MCF-7/MXcells are Sensitive to mitoxantrone fowllowing the chrysin treatment. The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was not significantly changed by only Chrysin treatment,so the chrysin can only become chemotherapy sensitizer.