Consider Chrysin. “so the chrysin can only become chemotherapy sensitizer.”
Chrysin Reverse the Drug Resistance by Targeting Breast Cancer Stem Cell Related Subpopulation
In the presence of chrysin (2.5Î¼M,5Î¼M), the IC50 value of mitoxantrone in MCF-7 cells was not significantly changed .Conclusions: 1ã€The MCF-7/MX cell line can be constructed by stepwise selection in mitoxantrone . 2ã€After being induced by mitoxantrone, MCF-7/MX cells is highly drug-resistant .3ã€
Chrysin can reverse drug resistance of MCF-7/MX cells by targeting BCRP.
Part Two : Chrysin influences breast cancer stem cell Subgroup in MCF-7/MX cell lines
Objective: To Investigate the influence of chrysin on CD44~+CD24~- cell in MCF-7/MX cell linesMethods: We used FACSC and Cell immunofluorescence to analyse the percentage of CD44~+ CD24~- cells in MCF-7 and MCF-7/MX cell lines.Results: Nearly 95% cells of MCF-7/MX and MCF-7 cell is CD44~+CD24~+. The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was was not significantly changed by only Chrysin treatment relative to the CD44~+CD24~-cells in untreated cells (P > 0.05).The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was decreased by Chrysin and Mitoxantrone treatment relative to the CD44~+CD24~-cells found at 3.2% in untreated cells. In MCF-7/MX cells treated by Chrysin and Mitoxantrone, the CD44~+CD24~-cells population was 2.684±0.226%,1.907±0.179 %,1.414±0.112 % and 1.448±0.057% at 10-2Î¼Mã€10-1Î¼Mã€100Î¼Mã€101Î¼M Chrysin , respectively(P < 0.05).
The same results could be Investigate by using Cell immunofluorescence.Conclusions: MCF-7/MXcells are Sensitive to mitoxantrone fowllowing the chrysin treatment. The percentage of the CD44~+CD24~-cells in MCF-7/MX cells was not significantly changed by only Chrysin treatment,so the chrysin can only become chemotherapy sensitizer.