Dr. Weeks’ Comment: The power of seed nutrition just gets stronger and stronger. Eat the Seeds to survive and thrive!
Anti-inflammatory effects of the Nigella sativa seed extract, thymoquinone, in pancreatic cancer cells
Navdeep Chehl et al First published: 22 July 2009Full publication histor DOI: 1111/j.1477-2574.2009.00059.xView/save citation
ABSTRACT Inflammation has been identified as a significant factor in the development of solid tumour malignancies. We have recently shown that thymoquinone (Tq), the major constituent of Nigella sativa oil extract, induced apoptosis and inhibited proliferation in PDA cells. Tq also increased p21 WAF1 expression, inhibited histone deacetylase (HDAC) activity, and induced histone hyperacetylation. HDAC inhibitors have been shown to ameliorate inflammation-associated cancer. In this study, we evaluated the anti-inflammatory potential of Tq in PDA cells in comparison with that of a specific HDAC inhibitor, trichostatin A (TSA).
Results: Tq dose- and time-dependently significantly reduced PDA cell synthesis of MCP-1, TNF-α, interleukin (IL)-1β and Cox-2. At 24 h, Tq almost completely abolished the expression of these cytokines, whereas TSA had a less dramatic effect. Tq, but not TSA, significantly and dose-dependently reduced the intrinsic activity of the MCP-1 promoter. Tq also inhibited the constitutive and TNF-α-mediated activation of NF-κB in PDA cells and reduced the transport of NF-κB from the cytosol to the nucleus.
Conclusions: Our data demonstrate previously undescribed anti-inflammatory activities of thymoquinone (Tq) in PDA cells, which are paralleled by inhibition of NF-κB. Thymoquinone (Tq) as a novel inhibitor of proinflammatory pathways provides a promising strategy that combines anti-inflammatory and proapoptotic modes of action.