Dr. Weeks’ Comment: Bee venom is a highly potent substance with myriad clinical benefits. Now science is catching up with folk tales and research shows exciting promise.
Prostate. 2011 Jun 1;71(8):801-12. doi: 10.1002/pros.21296. Epub 2010 Nov 17.
Anti-cancer effect of bee venom in prostate cancer cells through activation of caspase pathway via inactivation of NF-ÎºB.
Park MH, Choi MS, Kwak DH, Oh KW, Yoon do Y, Han SB, Song HS, Song MJ, Hong JT.
College of Pharmacy and Medical Research Center, Chungbuk National University, Gaeshin-dong, Heungduk-gu, Cheongju, Chungbuk, Korea.
Bee venom has been used as a traditional medicine to treat arthritis, rheumatism, back pain, cancerous tumors, and skin diseases. However, the effects of bee venom on the prostate cancer and their action mechanisms have not been reported yet.
To determine the effect of bee venom and its major component, melittin on the prostate cancer cells, apoptosis is analyzed by tunnel assay and apoptotic gene expression. For xenograft studies, bee venom was administrated intraperitoneally twice per week for 4 weeks, and the tumor growth was measured and the tumor were analyzed by immunohistochemistry. To investigate whether bee venom and melittin can inactivate nuclear factor kappa B (NF-ÎºB), we assessed NF-ÎºB activity in vitro and in vivo.
RESULTS AND CONCLUSIONS:
Bee venom (1-10”‰µg/ml) and melittin (0.5-2.5”‰µg/ml) inhibited cancer cell growth through induction of apoptotic cell death in LNCaP, DU145, and PC-3 human prostate cancer cells. These effects were mediated by the suppression of constitutively activated NF-ÎºB. Bee venom and melittin decreased anti-apoptotic proteins but induced pro-apoptotic proteins. However, pan caspase inhibitor abolished bee venom and melittin-induced apoptotic cell death and NF-ÎºB inactivation. Bee venom (3-6”‰mg/kg) administration to nude mice implanted with PC-3 cells resulted in inhibition of tumor growth and activity of NF-ÎºB accompanied with apoptotic cell death. Therefore, these results indicated that bee venom and melittin could inhibit prostate cancer in in vitro and in vivo, and these effects may be related to NF-ÎºB/caspase signal mediated induction of apoptotic cell death.