Niacinamide helps lessen Anxiety

Dr. Weeks’ Comment: For more than 3 decades I have helped people get off addictive pharmaceutical drugs like Valium (diazepam) and Clonazepam and the SSRI and SSNI anti-depressants which are biochemically best equated to dissociative anesthetic agents which disrupt sleep and create fugue-like zombie trances. One powerful ally in this fight to restore biochemical integrity in anxious or depressed people is vitamin B3 in the niacinamide form aka “nicotinamide” (niacin, the other vitamin B3 version, is best used for support cardiac health). Here is an exciting article on this vitamin.

These results suggest that while nicotinamide may exert some neuronal depressant and anxiolytic activity…”

Br J Pharmacol. 1985 Mar; 84(3): 689–696. doi: 10.1111/j.1476-5381.1985.tb16151.xPMCID: PMC1987159PMID: 2985162

Central effects of nicotinamide and inosine which are not mediated through benzodiazepine receptors.

J. M. BoldC. R. Gardner, and  R. J. Walker


The actions of nicotinamide and inosine were investigated on rat cerebellar Purkinje cells using ionophoretic and extracellular recording techniques. Ionophoretic application of nicotinamide or inosine showed that they were potent inhibitors of Purkinje cell firing. This inhibition differed from that induced by benzodiazepines in that it was not reversed by the GABA antagonists bicuculline methiodide and picrotoxin. RO 15-1788, the specific benzodiazepine antagonist, did not reverse the effects of nicotinamide. Chlordiazepoxide has been shown to increase significantly social interaction between pairs of male rats and this increase can be reversed by RO 15-1788, 20 mg kg-1 i.p. Nicotinamide also caused a small increase in social interaction but this effect was not reversed by the benzodiazepine antagonist. Inosine did not increase social interaction. [3H]-flunitrazepam binding studies showed that nicotinamide and inosine have only low affinities for the benzodiazepine binding site. These results suggest that while nicotinamide may exert some neuronal depressant and anxiolytic activity, its site of action appears not to be associated with the benzodiazepine receptor site. Similarly, inosine exerts a neuronal depressant effect dissimilar from that of benzodiazepines.

Full text

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • BALZER H, HOLTZ P, PALM D. [Studies on the biochemical principles of the convulsive effect of hydrazine]. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1960;239:520–552. [PubMed] [Google Scholar]
  • Crawley JN, Marangos PJ, Paul SM, Skolnick P, Goodwin FK. Interaction between purine and benzodiazepine: Inosine reverses diazepam-induced stimulation of mouse exploratory behavior. Science. 1981 Feb 13;211(4483):725–727. [PubMed] [Google Scholar]
  • File SE. The use of social interaction as a method for detecting anxiolytic activity of chlordiazepoxide-like drugs. J Neurosci Methods. 1980 Jun;2(3):219–238. [PubMed] [Google Scholar]
  • File SE, Hyde JR. Can social interaction be used to measure anxiety? Br J Pharmacol. 1978 Jan;62(1):19–24. [PMC free article] [PubMed] [Google Scholar]
  • Gerber GB, Deroo J. Metabolism of labeled nicotinamide coenzyme in different organs of mice and rats. Proc Soc Exp Biol Med. 1970 Jul;134(3):689–693. [PubMed] [Google Scholar]
  • Hunkeler W, Möhler H, Pieri L, Polc P, Bonetti EP, Cumin R, Schaffner R, Haefely W. Selective antagonists of benzodiazepines. Nature. 1981 Apr 9;290(5806):514–516. [PubMed] [Google Scholar]
  • Lapin IP. Nicotinamide, inosine and hypoxanthine, putative endogenous ligands of the benzodiazepine receptor, opposite to diazepam are much more effective against kynurenine-induced seizures than against pentylenetetrazol-induced seizures. Pharmacol Biochem Behav. 1981 May;14(5):589–593. [PubMed] [Google Scholar]
  • Marangos PJ, Martino AM, Paul SM, Skolnick P. The benzodiazepines and inosine antagonize caffeine-induced seizures. Psychopharmacology (Berl) 1981;72(3):269–273. [PubMed] [Google Scholar]
  • Möhler H, Okada T. Properties of 3H-diazepam binding to benzodiazepine receptors in rat cerebral cortex. Life Sci. 1977 Jun 15;20(12):2101–2110. [PubMed] [Google Scholar]
  • Möhler H, Polc P, Cumin R, Pieri L, Kettler R. Nicotinamide is a brain constituent with benzodiazepine-like actions. Nature. 1979 Apr 5;278(5704):563–565. [PubMed] [Google Scholar]
  • Salmoiraghi GC, Weight F. Micromethods in neuropharmacology: an approach to the study of anesthetics. Anesthesiology. 1967 Jan-Feb;28(1):54–64. [PubMed] [Google Scholar]
  • Skolnick P, Marangos PJ, Syapin P, Goodwin FK, Paul SM. CNS benzodiazepine receptors: physiological studies and putative endogenous ligands. Pharmacol Biochem Behav. 1979 May;10(5):815–823. [PubMed] [Google Scholar]
  • Slater P, Longman DA. Effects of diazepam and muscimol on GABA-mediated neurotransmission: interactions with inosine and nicotinamide. Life Sci. 1979 Dec 3;25(23):1963–1967. [PubMed] [Google Scholar]
  • Squires RF, Brastrup C. Benzodiazepine receptors in rat brain. Nature. 1977 Apr 21;266(5604):732–734. [PubMed] [Google Scholar]

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