Dr. Weeks’ Comments: Not only good for your teeth, xylitol is also anti-inflammatory!
“…These findings suggest that xylitol acts as an anti-inflammatory agent in THP-1-derived macrophages infected with live P. gingivalis, which supports its use in periodontitis…”
Xylitol, an Anticaries Agent, Exhibits Potent Inhibition of Inflammatory Responses in Human THP-1-derived Macrophages Infected With Porphyromonas Gingivalis
Eunjoo Park 1, Hee Sam Na, Sheon Min Kim, Shannon Wallet, Seunghee Cha, Jin Chung
Background: Xylitol is a well-known anticaries agent and has been used for the prevention and treatment of dental caries. In this study, the anti-inflammatory effects of xylitol are evaluated for possible use in the prevention and treatment of periodontal infections.
Methods: Cytokine expression was stimulated in THP-1 (human monocyte cell line)-derived macrophages by live Porphyromonas gingivalis, and enzyme-linked immunosorbent assay and a commercial multiplex assay kit were used to determine the effects of xylitol on live P. gingivalis-induced production of cytokine. The effects of xylitol on phagocytosis and the production of nitric oxide were determined using phagocytosis assay, viable cell count, and Griess reagent. The effects of xylitol on P. gingivalis adhesion were determined by immunostaining, and costimulatory molecule expression was examined by flow cytometry.
Results: Live P. gingivalis infection increased the production of representative proinflammatory cytokines, such as tumor necrosis factor-α and interleukin (IL)-1β, in a multiplicity of infection- and time-dependent manner. Live P. gingivalis also enhanced the release of cytokines and chemokines, such as IL-12 p40, eotaxin, interferon γ-induced protein 10, monocyte chemotactic protein-1, and macrophage inflammatory protein-1. The pretreatment of xylitol significantly inhibited the P. gingivalis-induced cytokines production and nitric oxide production. In addition, xylitol inhibited the attachment of live P. gingivalis on THP-1-derived macrophages. Furthermore, xylitol exerted antiphagocytic activity against both Escherichia coli and P. gingivalis.
Conclusion: These findings suggest that xylitol acts as an anti-inflammatory agent in THP-1-derived macrophages infected with live P. gingivalis, which supports its use in periodontitis.
Keywords: Cytokines; Porphyromonas gingivalis; inflammation; periodontitis; xylitol.
- Xylitol inhibits inflammatory cytokine expression induced by lipopolysaccharide from Porphyromonas gingivalis.Han SJ, Jeong SY, Nam YJ, Yang KH, Lim HS, Chung J.Clin Diagn Lab Immunol. 2005 Nov;12(11):1285-91. doi: 10.1128/CDLI.12.11.1285-1291.2005.PMID: 16275942 Free PMC article.
- Blocking proinflammatory cytokine release modulates peripheral blood mononuclear cell response to Porphyromonas gingivalis.Berker E, Kantarci A, Hasturk H, Van Dyke TE.J Periodontol. 2013 Sep;84(9):1337-45. doi: 10.1902/jop.2012.120422. Epub 2012 Nov 23.PMID: 23173823 Free PMC article.
- Porphyromonas gingivalis lipopolysaccharide alters atherosclerotic-related gene expression in oxidized low-density-lipoprotein-induced macrophages and foam cells.Lei L, Li H, Yan F, Li Y, Xiao Y.J Periodontal Res. 2011 Aug;46(4):427-37. doi: 10.1111/j.1600-0765.2011.01356.x. Epub 2011 Mar 21.PMID: 21418223
- By passing microbial resistance: xylitol controls microorganisms growth by means of its anti-adherence property.Ferreira AS, Silva-Paes-Leme AF, Raposo NR, da Silva SS.Curr Pharm Biotechnol. 2015;16(1):35-42. doi: 10.2174/1389201015666141202104347.PMID: 25483720 Review.
- Immunomodulation of macrophage functions by opioids.Gomez-Flores R, Weber RJ.Adv Exp Med Biol. 1998;437:13-9. doi: 10.1007/978-1-4615-5347-2_2.PMID: 9666252 Review. No abstract available.